Journal article
Comparison of contemporary invasive and non-invasive Streptococcus pneumoniae isolates reveals new insights into circulating anti-microbial resistance determinants
C Higgs, LS Kumar, K Stevens, J Strachan, T Korman, K Horan, D Daniel, M Russell, CA McDevitt, NL Sherry, TP Stinear, BP Howden, CL Gorrie
Antimicrobial Agents and Chemotherapy | AMER SOC MICROBIOLOGY | Published : 2023
DOI: 10.1128/aac.00785-23
Abstract
Streptococcus pneumoniae is a major human pathogen with a high burden of disease. Non-invasive isolates (those found in non-sterile sites) are thought to be a key source of invasive isolates (those found in sterile sites) and a reservoir of antimicrobial resistance (AMR) determinants. Despite this, pneumococcal surveillance has almost exclusively focused on invasive isolates. We aimed to compare contemporaneous invasive and non-invasive isolate populations to understand how they interact and identify differences in AMR gene distribution. We used a combination of whole-genome sequencing and phenotypic anti-microbial susceptibility testing and a data set of invasive (n = 1,288) and non-invasiv..
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Awarded by State Government of Victoria
Funding Acknowledgements
We thank all microbiologists from the laboratories in the state of Victoria for sending invasive isolates of <ITALIC>Streptococcus pneumoniae</ITALIC> to the Microbiological Diagnostic Unit (MDU) and supporting invasive pneumococcal disease (IPD) surveillance in the state. We gratefully acknowledge the staff of the Monash Health Microbiology Laboratory, who contributed non-invasive isolates to the study. We also thank the laboratories that participated in the Controlling Superbugs State-wide Snapshot project, which was funded by the Melbourne Genomics Health Alliance (supported by the State Government of Victoria). We also thank all staff at the Department of Health, Victoria, for their coordination and funding of IPD surveillance. Additionally, we wish to thank the AMR laboratory staff of the MDU Public Health Laboratory, and Controlling Superbugs project team members Dr. Diane Daniels, Dr. Chris Connor, Madeline Russell, and Sandra Johnson for their contributions.r MDU PHL is funded by the Victorian Government, Australia. This work was supported by NHMRC Partnership Project (GNT1149991). BPH is supported by NHMRC Investigator Grants (GNT1196103). The state-wide AMR snapshot was carried out as part of the 'Controlling Superbugs 2' study, funded by the Melbourne Genomics Health Alliance (supported by the State Government of Victoria).r C.H.: conceptualisation, data curation, analysis and data visualisation, writing - original draft. L.S.K.: resources, data curation. K.S.: data curation, writing - review and editing. J.S.: data curation, resources. N.L.S.: conceptualisation, writing - review and editing. K.H.: methodology, data analysis. D.D.: resources, data curation. M.R.: resources, data curation. J.Z.: methodology, data analysis. C.M: writing - review and editing. T.P.S.: conceptualisation, writing - review and editing. B.P.H.: conceptualisation, writing - review and editing. C.L.G.: conceptualisation, writing - review and editing.